The PacBio Iso-Seq allows sequencing of transcript isoforms in their entirety, with no assembly required by leveraging the long reads to deliver a single, highly accurate sequence for each transcript isoform. The Iso-Seq bioinformatics pipeline processes the data into high-quality consensus transcript sequences enabling accurate isoform annotation and open reading frame prediction. These features do make Iso-Seq the method of choice for example for de novo gene annotations.

TGIA provides total solutions for Iso-Seq that compromise RNA prep, library prep, SMRT sequencing, and bioinformatics analysis.