Whole genome sequencing for de novo assembly using SMRT Sequencing generates contiguous and highly accurate reference genomes. With megabase-size contig N50s, consensus accuracies >99%, and tools for phasing haplotypes, PacBio assemblies capture undetected SNPs, fully intact genes, and regulatory regions embedded in complex structures that fragmented draft genomes often miss.
TGIA’s standard library prep offers an approximately 20 kb, size-selected library from high-quality genomic DNA to maximize genome contiguity.